Table 6.
Primers used for MLST
| Locus | Putative function | Locationa | Forward primer | Reverse primer | Length (bp) |
|---|---|---|---|---|---|
| gyrB | DNA gyrase subunit B | 6,031-7,971 | TGGTGCATCGGTAGTTAATGC | CAACATCTGGGTTTTCCATCAT | 657 |
| dapE | Succinyl diaminopimelate desuccinylase | 301,402-302,538 | GTAAATATTGATTCGACTAATG | CACTAGCACTTGTTTCACTG | 669 |
| hisJ | Histidinol phosphate phosphatase | 606,408-607,235 | TCCACATGGTACGCATGAT | GGACATGTCAAAATGAAAGATC | 714 |
| sigB | Stess responsive alternative sigma factor B | 924,734-925,513 | CCAAAAGTATCTCAACCTGAT | CATGCATTTGTGATATATCGA | 642 |
| ribC | Riboflavin kinaseand FAD synthase | 1,364,536-1,365,480 | AAGACGATATACTTACATCAT | GTCTTTTTCTAACTGAGCA | 633 |
| purM | Phosphoribosyl aminoimidazole synthase | 1,893,107-1,894,153 | CAAGCTCCACTTTGACAGCTAA | TAAAGCAGGCGTGGACGTA | 693 |
| betL | Glycine betaine transporter | 2,216,882-2,218,405 | ACAGAACATTATCCAAATGAGTT | ACGTTGTGATTTTTTCGGTC | 534 |
| gap | Glyceraldehyde 3-phosphate dehydrogenase | 2,578,558-2,579,584 | CTGGATCAGAAGCTGCTTCCA | GTCGTATTCAAAATGTGGAAGGA | 621 |
| tuf | Translation elongation factor | 2,816,958-2,818,145 | CATTTCTACTCCAGTTACTACT | GCTCTAAACCCCATGTTA | 681 |
| Subtotal | 5,844 |
a, Positions correspond to complete genome sequence of L. innocua strain CLIP11262 (AL592022).