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. 2010 May 11;5(5):e10575. doi: 10.1371/journal.pone.0010575

Figure 1. Ectopic expression of TRIM25 restores IFN induction in Huh7.25.CD81 cells.

Figure 1

Huh7, Huh7.5 and Huh7.25.CD81 cells were transfected with the pGL2-IFNβ-FLUC/pRL-TK-RLUC reporter plasmids alone or the in presence of plasmids expressing RIG-I (200 ng), TRIM25 (100 ng) or MAVS (400 ng). 24 hrs post transfection, cells were mock-infected or infected with Sendaï virus (SeV) (40 HAU/ml). 24 hrs after infection, luciferase activity was measured and F-luc was normalized against R-luc. IFN expression was expressed as fold induction over control cells that were simply transfected with pGL2-IFNβ-FLUC/pRL-TK-RLUC. Error bars represent the mean ± S.D. for triplicates.