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. 2010 May 11;5(5):e10575. doi: 10.1371/journal.pone.0010575

Figure 2. Kinetics of MAVS cleavage in Huh7.25.CD81 cells after JFH1 infection.

Figure 2

Huh7.5 cells and Huh7.25.CD81 cells were transfected with an HA-TRIM25 expressing plasmid or with an empty plasmid. 24 hrs post-transfection, cells were mock-infected or infected with JFH1 (m.o.i = 0.05) for the indicated times and cell lysates were generated. Cell extracts (50 µg) were subjected to SDS-12.5% PAGE and blotted with anti-MAVS, anti-NS3, anti-HA or anti-actin as indicated. The arrows indicate the position of full-length MAVS and MAVS cleaved in the presence of HCV NS3/4A.