Figure 4. A comparison of eye size in MO1 morpholino-injected larvae and control-injected larvae.

Graph shows mean eye size (measured in µm on y-axis) for MO1 morpholino-injected larvae (MO1; light purple) compared to control-injected larvae (Control MO; dark purple) at three different time periods, 2 dpf, 4 dpf and 6 dpf, labeled on the x-axis. Data are shown as mean +/− one standard deviation, measuring a minimum of 9–20 independent retinas per data point. Analysis using a Two-sample t-test assuming equal variance showed a significant difference at 2 dpf, 4 dpf and 6 dpf (p<0.05 for 2 dpf, 4 dpf and 6 dpf).