Table 2. Frequency of Microphthalmia and/or Coloboma with 6 ng MO1 Morpholino and 100 pg human TMX3 wild type or 100 pg of human TMX3/(p.Arg39Gln).
MO1 Morpholino (n = 349) | MO1 Morpholino +100 pg Human TMX3 wild type (n = 582) | MO1 Morpholino +100 pg Human TMX3/(p.Arg39Gln) (n = 440) | |
Microphthalmia/Colobomaa | 18 (5.2%) | 7 (1.2%)c | 30 (6.8%)d |
External Toxicityb | 46 (13.2%) | 164 (28.18%) | 77 (17.5%) |
Normal | 285 (81.6%) | 411 (70.62%) | 333 (75.7%) |
Microphthalmia/Colobomaa: Larvae with microphthalmia or coloboma only and without any other evidence external toxicity.
External toxicityb: Edema, body axis curving; can also include eye defects.
: P = 0.0031 for the frequency of microphthalmia and/or coloboma using MO1 morpholino versus frequency of microphthalmia and/or coloboma using MO1 morpholino +100 pg Human TMX3 wild type. Fish with external toxicity were excluded from the analysis. A two-tailed Fisher's exact test was used.
: P = 0.293 for the frequency of microphthalmia and/or coloboma using MO1 morpholino versus frequency of microphthalmia and/or coloboma using MO1 morpholino +100 pg Human TMX3(p.Arg39Gln). Fish with external toxicity were excluded from the analysis. A two-tailed Fisher's exact test was used.