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. 2010 Apr 27;10:160. doi: 10.1186/1471-2407-10-160

Table 1.

Primer pairs used for generating NGX6 promoter construct pGL3 -357/+769, pGL3-357/+276, pGL3 +276/+769, pGL3 -357/-159, pGL3 -159/+276.

pGL3 -357/+769 Forward: 5'-aaaggtaccCGAGCCCAGAGGGTTTACTT-3'
Reverse: 5'-cccaagcttGCCTCAATCTTCCCTGCTTC-3'
pGL3 -357/+276 Forward: 5'-aaaggtaccCGAGCCCAGAGGGTTTACTT-3'
Reverse: 5'-cccaagcttGGGGATTGGGATAGGACGAG-3'

pGL3 +276/+769 Forward: 5'-aaaggtaccCTCGTCCTATCCCAATCCCC-3'
Reverse: 5'-cccaagcttGCCTCAATCTTCCCTGCTTC-3'

pGL3 -357/-159 Forward: 5'-aaaggtaccCGAGCCCAGAGGGTTTACTT-3'
Reverse: 5'-cccaagcttCAAGCACAGAGCCCGAGGTC-3'

pGL3 -159/+276 Forward: 5'-aaaggtaccGGGGTGAGAAAGGCAGGGTC-3'
Reverse: 5'-cccaagcttGGGGATTGGGATAGGACGAG-3'