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. 2010 May 11;5(5):e10589. doi: 10.1371/journal.pone.0010589

Figure 1. Syn-GFP is present in neuronal cell bodies and presynaptic terminals.

Figure 1

A. Fixed tissue confocal image of Syn-GFP in the cortex shows one neuronal soma and that the vast majority of neuropil puncta staining is present within axons. Endogenous GFP fluorescence is shown without antibody labeling. B. In vivo multiphoton image of Syn-GFP in the cortex shows staining in one neuronal soma and multiple neuropil puncta. Scale bar for A–B 10 µm. C. Fixed tissue confocal image from the cortex of a Syn-GFP animal showing GFP staining in two somata and in the neuropil. D. Staining in the same section for human α-synuclein. E. Merged image shows colocalization between GFP signal and human α-synuclein, as expected for this fusion protein. Scale bar for C–E 20 µm.