| Wherein: | |
| X | = the mean fluorescence intensity per bead region per well |
| tα/2 | = 2-tailed t distribution, for a specified level of confidence (α) |
| n-1 | = degrees of freedom (df), sampled bead count per region per well minus one |
| σ | = standard deviation of the fluorescence intensity per bead region per well |
| n | = sample size, the number of beads sampled per bead region per well |
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