Fluoxetine (10 mg/kg, b.i.d.) exposure during adolescence regulates anxiety-like behavior in the EPM (A and B). Short-term (n = 8/group): FLX significantly reduced time spent (A, left panel) and entries (B, left panel) into the open arms of the EPM 24 hours after the last FLX injection (p < .05). Long-term (n = 8/group): FLX also reduced time spent (A, right panel) in the open arms of the EPM, without influencing entries (B, right panel) compared with VEH-treated control rats. Data are presented as percent time spent and percent entries (mean ± SEM) into the open arms of the EPM. b.i.d., twice daily; EPM, elevated plus-maze; FLX, fluoxetine; VEH, vehicle.