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Effect of centrifugation on a LBC. A small segment of a LBC of the newt N. viridescens, from a preparation that had been centrifuged to attach the chromosomes to a glass microscope slide. Note that all the lateral loops of the chromosome are in essentially the same plane. This image was taken after immunofluorescent staining with an antibody against an abundant chromosomal protein. Bar = 50 μm.
