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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Methods. 2010 Feb 1;51(1):101–105. doi: 10.1016/j.ymeth.2010.01.031
6 μl XTL
0.6 μl CTX [1M KCl, 16 mM Mg(CH3COO)2, 20 mM DTT]
1 μl CPK [200 mM creatine phosphate, 4 mg/ml creatine kinase (Sigma)]
0.2 μl amino acid mix minus Met (Promega)
10 μCi [35S]-methionine (EasyTag, Perkin Elmer)
0.5 μl recombinant RNasin (Promega)
1 μl DEPC-dH2O