Preparation of a highly active cell-free translation system from immature Xenopus laevis oocytes

. Author manuscript; available in PMC: 2011 May 1.

Published in final edited form as: Methods. 2010 Feb 1;51(1):101–105. doi: 10.1016/j.ymeth.2010.01.031

6 μl	XTL
0.6 μl	CTX [1M KCl, 16 mM Mg(CH₃COO)₂, 20 mM DTT]
1 μl	CPK [200 mM creatine phosphate, 4 mg/ml creatine kinase (Sigma)]
0.1 μl	amino acid mix minus Leu (Promega)
0.1 μl	amino acid mix minus Met (Promega)
0.7 μl	DEPC-dH₂O
0.5 μl	recombinant RNasin (Promega)
1 μl	luciferase reporter RNA [75nm (see section 2.1.4)]