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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Biochemistry. 2010 May 18;49(19):4227–4234. doi: 10.1021/bi1001823

Figure 4.

Figure 4

Western blot of extracts of mitochondria from cells grown on glucose (left lane), galactose (middle) and glycerol (right) (Samples F5, RF1 and R1(18)). For each sample, 60 μg of protein, determined by the bicinchoninic acid method (Thermo Scientific), was added to each lane of a 10% SDS-PAGE gel. Primary antibodies used in staining included Kar2p, CPY, PGK, and porin. Integrated intensities of contaminating bands were normalized to the intensity of the corresponding porin band. These ratios are given in the table along with the percentage of the NHHS FeII doublet observed in the Mössbauer spectrum of the same material.