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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Methods. 2010 Jan 22;51(1):152–156. doi: 10.1016/j.ymeth.2010.01.023

Figure 1. Monitoring the translational behavior of Xenopus mRNAs by polyribosome analysis.

Figure 1

Extacts prepared from oocytes, eggs or are fractionated using sucrose step gradients. During centrifugation the non-polyribosomal mRNAs remain in the supernatant fraction while the polyribosome associated mRNAs partition to the bottom of the tube. RNAs extracted from the non-polyribosomal and polyribosomal fractions are analyzed using quantitative methods to determine whether specific mRNAs are polyribosome associated and therefore translationally active.