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. 2010 Jan 22;86(3):374–382. doi: 10.1093/cvr/cvq023

Figure 5.

Figure 5

ENH1 silencing in neonatal rat cardiomyocytes. To silence the expression of ENH1, specific shRNA were delivered into ventricular neonatal rat cardiomyocytes by adenoviruses. Cardiomyocytes were first treated with ENH1-RNAi for 48 h and then stimulated with phenylephrine (20 µM), aldosterone (1 µM), or endothelin I (10 nM) for 30 h. The quantitative amount of ENH1 mRNA was determined by real-time PCR using 50 ng of total RNA. Data shown are means and SD of three independent measurements from three independent cell preparations (*P < 0.0002 and **P< 0.0001).