Table 1.
Primers and probes sequences used for real-time PCR
| Forward 5′–3′ | Reverse 5′–3′ | Probe 5′–3′ | |
|---|---|---|---|
| Rat ENH1 | GGC TCC ACC TGG CAT GAC | AAA ATG TCT GCC CTT CCA AAC TT | FAM-TTG CTT TGT GTG CTC CGT GTG TTG TG-BHQ1/TAMRA |
| Rat ENH3 | ACC AGC GTG TGT TCC GAG T | CGT TTG GGT GGA ATT TTC ACA | FAM-CAG CAA AAT GGT AAC CCT GGC A-BHQ1/TAMRA |
| Rat ENH4 | GCT GTC CAG AAG AAA GCA CA | TGT GAT AAA ACT CCG TGT TGC | FAM-ACC CAA ACG CCC ACC AAG AA-BHQ1/TAMRA |
| Rat cyclophilin A | TCT GGG ATA CAC TTG GCA TGA T | TCA AAT TTC TCT CCG TAG ATG GAC TT | FAM-ACA CGC CAT AAT GGC ACT GGT GG-BHQ1/TAMRA |
| Mouse ENH1 | TCT GGG ATA CAC TTG GCA TGA T | GCC CTT CCA AAC TTT CAC AAC A | FAM-CTT GCT TTG TGT GCT CTG-BHQ1/TAMRA |
| Mouse ENH3 | AGA GGA TCC CAG GGT GAC ATT A | CGT TTA GGT GGA ATT TTC ACA | FAM-CAG CAA AAT GGT AAC CCT GGC A-BHQ1/TAMRA |
| Mouse ENH4 | CCG TCC AGA AGA AAA CAC AC | TGT GAT AAA ACT CCG TGT TGC | FAM-CCA CCT AAA CGC CCA CCA AGA-BHQ1/TAMRA |
| Mouse cyclophilin A | TGT GCC AGG GTG GTG ACT T | TCA AAT TTC TCT CCG TAG ATG GAC CT | FAM-ACA CGC CAT AAT GGC ACT GGC GG-BHQ1/TAMRA |
| Rat ANP | ATG GGC TCC TTC TCC ATC AC | CAT CTT CTC CTC CAG GTG GTC | |
| Rat BNP | GGG CTG TGA CGG GCT GAG GTT | AGT TTG TGC TGG AAG ATA AGA | |
| Rat betaMHC | GCA GCT TAT CAG GAA GGA ATA C | CTT GCG TAC TCT GTC ACT C |