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Mutations Introduced to Make RNase Sa 8S and Their Expected Contribution to Stability
| Type of mutation | Mutation | ΔTm (°C)a | ΔTm (°C)b (observed) | ΔΔGa | ΔΔG°b (observed) |
|---|---|---|---|---|---|
| Surface charge | D25K | 3.0 | — | 0.9 | — |
| E74K | 3.9 | — | 1.1 | — | |
| Additive | 2S | 6.9 | 2.2 | 2.0 | 0.8 |
| β-Turn | S31P | 2.5 | — | 0.7 | — |
| S42G | 2.6 | — | 0.7 | — | |
| S48P | 4.5 | — | 1.3 | — | |
| T76P | 3.5 | — | 1.0 | — | |
| Q77G | 2.9 | — | 0.8 | — | |
| Additive | — | 16.0 | 15.1 | 4.5 | 4.5 |
| Subtotal | 7S | 22.9 | 17.3 | 6.5 | 5.3 |
| Buried charge | D79F | 9.9 | 10.6 | 3.0 | 2.4 |
| Total | 8S | 32.8 | 27.9 | 9.5 | 7.7 |
