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. 2010 Mar 26;19(5):1065–1078. doi: 10.1002/pro.387

Figure 2.

Figure 2

Purification of the synthetic N-DmI, R-DmI, and Biotin-N-DmI. A: RP-HPLC analysis of crude peptides. B: Purity check of HPLC-purified peptides. The column was eluted with a linear acetonitrile gradient (---) in 0.1% aqueous TFA at a flow rate of 0.8 mL min−1. The peptide material corresponding to the major peaks in the chromatograms was collected and subjected to MS analysis.