Figure 3.

Assignment of disulfide pairing of the synthetic N-DmI by enzymatic peptide mass fingerprint analysis. A: RP-HPLC analysis of the proteolysis reaction with trypsin. B: RP-HPLC analysis of the proteolysis reaction with chymotrypsin. The chemical identity of tryptic (T) and chymotryptic (C) fragments was established by MS analysis, as reported in Supporting Information Table S1. C: Amino acid sequence and disulfide bond topology of the synthetic N-DmI, as deduced from MS data reported in Supporting Information Table S1. Only the peptide fragments containing a single disulfide bond (---) are indicated.