Table I.
Amp Resistance Conferred by Different Target Proteins
| Protein-of-interesta | Strain | MBC (μg/mL)b | MIC (μg/mL)b |
|---|---|---|---|
| Alkaline phosphatase | |||
| PhoA | DHB4 | 200 | 200 |
| PhoA | DHA | 25 | 25 |
| PhoA[SSCC] | DHB4 | 400 | 200 |
| ssTorA-PhoA-Bla | DHB4 | 6 | 6 |
| ssTorA-PhoA-Bla | DHA | 12 | 6 |
| Bla | DHB4 | 3200 | 1600 |
| Bla | DHA | 3200 | 1600 |
| Anti-β-gal scFv13 | |||
| scFv13 (wild type) | DHB4 | 400 | 200 |
| scFv13 (wild type) | DHA | 50 | 50 |
| scFv13-R4 | DHB4 | 400 | 400 |
| scFv13-R4 | DHA | 100 | 50 |
| Maltose-binding proteins | |||
| MalE (wild type) | DH5α | 250 | 200 |
| G32D | DH5α | 200 | 175 |
| I33P | DH5α | 200 | 175 |
| MalE31 | DH5α | 175 | 175 |
| Other proteins | |||
| GFP | DH5α | 200 | 175 |
| GST | DH5α | 50 | 25 |
| MetF | DH5α | 25 | 25 |
| MetK | DH5α | 6 | 3 |
| TrxA | DH5α | 100 | 100 |
| Amyloid-β peptides | |||
| Aβ42 (wild type) | DH5α | 12 | 12 |
| A2 | DH5α | 25 | 25 |
| A4 | DH5α | 25 | 25 |
| B9 | DH5α | 25 | 25 |
| B12 | DH5α | 25 | 25 |
| H2 | DH5α | 50 | 25 |
| GM6 | DH5α | 100 | 100 |
| GM7 | DH5α | 100 | 50 |
| GM11 | DH5α | 100 | 50 |
a
Unless otherwise indicated, protein-of-interest is a fusion of ssDsbA-POI-Bla.
b
Approximately 500 CFUs were plated overnight at 37°C.