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. Author manuscript; available in PMC: 2010 May 12.
Published in final edited form as: Nano Res. 2008 Dec;1(6):502–518. doi: 10.1007/s12274-008-8054-z

Figure 8.

Figure 8

SDS PAGE analysis of time studies. (a) Uptake time course. Lane 1 hGH control; Lane 2 NIPAm/CB particle supernatant after 1 min incubation (Out); Lane 3 NIPAm/CB particle content after 1 min incubation (In); Lane 4 NIPAm/CB particle supernatant after 5 min incubation (Out); Lane 5 NIPAm/CB particle content after 5 min incubation (In); Lane 6 NIPAm/CB particle supernatant after 20 min incubation (Out); Lane 7 NIPAm/CB particle content after 20 min incubation (In); Lane 8 NIPAm/CB particle supernatant after 40 min incubation (Out); Lane 9 NIPAm/CB particle content after 40 min incubation (In); Lane 10 NIPAm/CB particle supernatant after 60 min incubation (Out); Lane 11 NIPAm/CB particle content after 60 min incubation (In). The sequestration process was very fast, with the majority of hGH being captured by particles after one minute. (b) Preservation time course. Lane 1 hGH control; Lane 2 NIPAm/CB particle supernatant after 1 h (Out); Lane 3 NIPAm/CB particle content after 1 h incubation (In); Lane 4 NIPAm/CB particle supernatant after 4 h incubation (Out); Lane 5 NIPAm/CB particle content after 4 h incubation (In); Lane 6 NIPAm/CB particle supernatant after 24 h incubation (Out); Lane 7 NIPAm/CB particle content after 24 h incubation (In); Lane 8 NIPAm/CB particle supernatant after 48 h incubation (Out); Lane 9 NIPAm/CB particle content after 48 h incubation (In). Once captured by particles, hGH remains stable up to at least 48 h