Table I. Summary of Sequence Data.

Summary of all TCRβ sequence data. NRP-V7-specific CD8⁺ T cells were single-cell sorted and their TCRβ genes amplified by RT-PCR and sequenced. Numbers of mice included, total sequences recovered, unique sequences recovered, and number of shared clones are listed for each group. A clonotype was defined as shared if it was recovered from more than one mouse. One mouse contributed more than one sample to this analysis (islets and PLNs taken from one 10 week old animal). Otherwise all mice contributed T cells derived from only one tissue.

Sample	# mice	# sequences	# unique clonotypes	# shared clonotypes
Islets 8–10 weeks	6	97	34	7 (20%)
Islets 12–14 weeks	5	148	14	10 (71%)
PLNs 8–10 weeks	3	61	36	6 (17%)
PLNs 12–14 weeks	2	68	34	8 (24%)
Spleen 8–10 weeks	2	55	41	6 (15%)
Spleen 12–14 weeks	3	134	72	6 (8%)