Table 2.

Ingredients and their relative proportions to prepare ChAT buffer substrate

Concentration in ChAT Buffer Substrate	Final concentration in assay
60 mM Sodium phosphate buffer, pH 7.4	42.9 mM
0.2 mM EDTA (tetrasodium)	0.14 mM
12 mM choline chloride	8.6 mM
0.12 mM eserine sulfate	0.086 mM
240 mM NaCl	171 mM
0.15 mM [14C]-acetylcoenzyme A	0.11 mM