Table 4.
Mutagenesis screens
| Expansion of families |
Phenotypic screens |
|||||||
|---|---|---|---|---|---|---|---|---|
| chimaera | no. G1 | no. G2 | pups/mating | no. G3 | vis. | fert. | eye | hear. |
| v6.4-C1 | 6 | 38 | 1.4 | 93 | 1 + ser (3) | 3/48 | 2/60 | 3(2)/69 |
| v6.4-C2 | 6 | 29 | 3.0 | 161 | 3 | 8/78 | 3/118 | 0/124 |
| v6.4-C3 | 1 | 3 | 0.0 | 0 | – | – | – | – |
| v6.4-C7 | 6 | 28 | 1.5 | 57 | 2 + Sne (3) | 1 + Rsph (2); Sgdp (2)/24 | 4/44 | 0/41 |
| CJ7-1 | 2 | 9 | 4.8 | 53 | 0 | Mei1 (5)/15 | 0/16 | 0/16 |
| CJ7-2 | 1 | 3 | 7.7 | 23 | 1 + Pde (5) | 0/4 | 0/16 | 1/18 |
| CT129-C | 7 | 35 | 6.0 | 265 | 1 + Twl (3) | 0/92 | 1/89 | 0/124 |
| CT129-D | 3 | 11 | 3.1 | 52 | 1 + Pfz (5) | 1/18 | 2/24 | 0/31 |
| Totals | 32 | 156 | 704 | 14 | 16/310 | 12/367 | 3/423 | |
The founder chimaeras are listed on the left, with the prefix designation reflecting the ES cells from which they were derived. ‘No. G2’ lists the total number of sons or daughters from all G1s that were backcrossed to a G1 parent. ‘Pups/mating’ is the total number of weaned offspring per mating period. A mating period consisted of at least one week in which a G1 and G2 were together in a cage before being separated. If two litters were produced in a mating, it was counted as two mating periods. G3 values indicate the number of pups weaned. All G3 animals were screened for visible mutations (‘vis.’); the number of variants not proven to be genetic is indicated and the names of proven mutations are indicated after the ‘+’, with the total number of animals exhibiting that mutation given in parentheses (‘ser’, a short-eared variant not discussed in the text). For the remaining phenotypic screens, the numerator is the number of variants identified and the denominator is the t otal number of animals examined. In those cases in which multiple animals in a family have the same heritable mutation, the number of such animals is given in parentheses, in some cases along with the mutant symbol. ‘Fert.’, infertile males; ‘hear.’, mice that failed both click box and ABR tests. The totals reflect the number of affected animals (in the numerator; underlined numbers indicate the adjusted number of independent phenotypes) over the total number of animals.