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. Author manuscript; available in PMC: 2010 May 12.
Published in final edited form as: Neurobiol Aging. 2008 Apr 10;31(1):58–73. doi: 10.1016/j.neurobiolaging.2008.03.001

Figure 4.

Figure 4

APP promoter sequences can induce the formation of extranuclear AFT complexes. (A) Co-transfection of Dig-labeled (red) promoter plasmids into cells transfected to generate AFT complexes induced the formation of extranuclear AFT complexes close to sites of labeled promoter plasmid (arrow). (B) Fe65 and Tip60 were transfected to generate AFT complexes and APP promoter plasmids were subsequently transfected 3 hours prior to fixation. Extranuclear AFT complexes can now be observed at greater distances from the nucleus (arrow) with nearby labeling of promoter DNA. (C) Extensive washing removes much of the extracellular promoter plasmid DNA, while extranuclear AFT complexes are still found to be associated with APP promoters. Bar, 5 μm.