Figure 5.
AFT complexes are closely associated with the chromosomal loci of AICD-regulated genes. Fluorescence in situ hybridization was performed on clonal cells transfected to generate AFT complexes. (A) Hybridization with a BAC containing sequences of the 3′ end of the APP gene. Close association of the FISH signal with an AFT spot is marked by an arrow. (B) FISH signals from a BAC containing sequences from the APP promoter are similarly associated with AFT complexes (arrows). (C) Hybridization with a BAC containing sequences of the KAI1 gene. Two of the labeled genomic loci are associated with AFT complexes. Bar, 7.5 μm.