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A. VGAT staining. Only the IPL stains above background, while the INL and OPL remain unstained.
B–D. Higher magnification of IPL (from a different section) stained for PKC (red) and VGAT (green). Rod bipolar descending axons (arrowhead) and their terminals (*) are devoid of VGAT staining. VGAT staining is found however around the axon terminals (arrows), presumably in GABAergic amacrine varicosities (imaged under 100x).
