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A. Double labeling for parvalbumin (PV, red) and GAT3 (green). Horizontal cell somas (h) stain for GAT3. (Imaged under 100x)
B. Triple staining for PKC+PV (red) and GAT3 (green). GAT3 labels both horizontal cells (h) and rod bipolar cells (r), but certain GAT3-positive cells belong to neither of these cell types (arrowhead lies inside the cell and points to the stained membrane) (imaged under 10x zoom 2).
