A: MCF7 cells transfecetd with vector only (V), wild type (S), or double mutant T457A,S459A CEACAM1-4S (DA) were grown in Matrigel for 2, 4, or 6d, cells harvested, protein separated by SDS gel electrohoresis, and western blotted with anti-calpain-9 or anti-β-actin antibodies. B: Same as above (day 4 only), cells treated with lipofectamine only, RNAi control oligo, PKC-δ RNAi oligo 1, or CAPN9 RNAi oligo 1 and western blotted with anti-calpain-9 or anti-β-actin antibodies.