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. Author manuscript; available in PMC: 2010 May 12.
Published in final edited form as: Exp Cell Res. 2009 Nov 10;316(4):638–648. doi: 10.1016/j.yexcr.2009.11.001

Figure 2. Expression of Calpain-9 protein in MCF7 cells transfected with vector, CEACAM1-4S wild type or double mutant T457A,S459A grown in Matrigel in the presence or absence of RNAi.

Figure 2

A: MCF7 cells transfecetd with vector only (V), wild type (S), or double mutant T457A,S459A CEACAM1-4S (DA) were grown in Matrigel for 2, 4, or 6d, cells harvested, protein separated by SDS gel electrohoresis, and western blotted with anti-calpain-9 or anti-β-actin antibodies. B: Same as above (day 4 only), cells treated with lipofectamine only, RNAi control oligo, PKC-δ RNAi oligo 1, or CAPN9 RNAi oligo 1 and western blotted with anti-calpain-9 or anti-β-actin antibodies.