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. Author manuscript; available in PMC: 2010 May 12.
Published in final edited form as: Exp Cell Res. 2009 Nov 10;316(4):638–648. doi: 10.1016/j.yexcr.2009.11.001

Figure 3. Inhibition of lumen formation of MCF7/CEACAM1-4S cells by calpain inhibitors calpectin and PD150606.

Figure 3

A-B: MCF7 cells transfected with wild type CEACAM1 were treated with 0.05% DMSO for 6d in Matrigel and examined at 50x (A) or 400x (B) by phase contrast microscopy. After examination of 200 acini, the percent lumen formation was 95% (Table 2), essentially identical to untreated controls (data not shown). C-D: Same as above treated with 10 μM calpectin in 0.05% DMSO and examined at 50x (C) or 400x (D) by phase contrast microscopy (15% lumen formation, Table 2). E-F: same as above treated with 20 μM PD150606 in 0.05% DMSO and examined at 50X (E) or 400X (F) by phase contrast microscopy (5% lumen formation, Table 2).