Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Nat Prod. 2009 Nov;72(11):1927–1936. doi: 10.1021/np900444m

Figure 1. Inhibition of HIF-1 activation.

Figure 1

(A) Concentration-dependent inhibitory effects exerted by 6 and 7 on hypoxia (1% O2)-induced HIF-1 activation in a T47D cell-based reporter assay (mean ± SD, one experiment in triplicate). (B) Compounds 5, 8, 13 and 22 were evaluated at 5.6, 10, 17.8, and 30 μM in the reporter assay described in (A). (C) Concentration-response results of 5 – 8, 13, and 22 on 1,10-phenanthroline (10 μM)-induced HIF-1 activation in a T47D cell-based reporter assay (mean ± SD, one experiment in triplicate). (D) Levels of secreted VEGF protein in the media conditioned by T47D cells exposed to hypoxia in the presence of 6 or 7 (1% O2, 16 h), were determined by ELISA and normalized to the amounts of cellular proteins (mean ± SD, one experiment in triplicate). The p value was provided when there was a statistically significant difference between the hypoxia-induced and the compound treated samples.