Figure 4.

LL-37 reduces high glucose–mediated delay in epithelial wound healing in porcine corneal organ culture. Epithelial wound of 5-mm diameter was made (A, original wound) in the center of porcine corneas and allowed to heal for 48 hours in MEM containing NG (B; 5 mM d-glucose), or high glucose (C; 25 mM d-glucose) in the presence of 0.2 μg/mL (D) or 0.5 μg/mL (E) LL-37. Organ-cultured corneas were stained with Richardson staining solution to show the remaining wounds. Micrographs represent 1 of 3 samples performed each time. Bar graph represents the statistical analysis of the extent of healing. Values are expressed as mean ± SEM. *P < 0.05 and **P < 0.01 (Student's t-test) compared with HG. Results are representative of five independent experiments.