Table 2.
Cell Densities Measured by the ConfoScan 4 and Tandem Scanning Confocal Microscopes in 16 Corneas
| Stromal Layer | ConfoScan 4 |
TSCM |
|||
|---|---|---|---|---|---|
| Automatic | Manual | Difference Auto − Manual | Automatic | Difference TSCM − ConfoScan 4 | |
| Anterior 10% | 31,677 ± 4,886 | 31,358 ± 5,506 | 319 ± 4,009 | 44,126 ± 6,680 | 12,449 ± 8,326* |
| 10%–33% | 25,890 ± 3,517 | 26,062 ± 4,046 | −172 ± 2,695 | 27,582 ± 4,553 | 1,692 ± 4,843 |
| 33%–66% | 24,419 ± 3,498 | 25,939 ± 2,828 | −1,520 ± 2,172† | 23,317 ± 5,043 | −1,103 ± 6,107 |
| 66%–90% | 24,493 ± 3,092 | 24,983 ± 2,171 | −490 ± 2,765 | 24,498 ± 3,475 | 5 ± 5,433 |
| 90%–100% | 22,728 ± 4,930 | 23,610 ± 3,096 | −883 ± 3,418 | 23,230 ± 4,798 | 502 ± 7,506 |
| Unweighted mean, n = 80 layers | 25,841 ± 5,027 | 26,391 ± 4,486 | −549 ± 3,065 | 24,647 ± 4,661 | 2,709 ± 8,114* |
| 274 ± 5,994‡ | |||||
Cell densities are expressed as the mean cells per cubic millimeter ± SD. These corneas were independent of corneas used to determine selection criteria for the automatic method. The differences were not significant (P > 0.1), except where indicated. TSCM, Tandem Scanning confocal microscope.
*
ConfoScan 4 significantly different from TSCM, P < 0.001, paired t-test.
†
Automatic significantly different from manual, P = 0.014, paired t-test.
‡
Mean difference between 10% and 100% stromal thickness.