Figure 2.

rmIL-33 protein treatment (A). Significantly lower clinical scores were seen at 1, 3, and 5 dpi in rmIL-33 compared with PBS mice (P < 0.01, for each). The experiment was performed twice with 5 mice/group/time, and data shown are individual scores from both experiments. Photographs taken with a slit lamp at 5 dpi show less severe disease after rmIL-33 treatment (B) compared with corneal thinning or perforation in PBS-treated controls (C). Plate counts to enumerate viable bacteria (D) and MPO assay to quantitate corneal PMN (E) showed fewer bacteria and PMN in the cornea of rmIL-33 than of PBS-treated mice at 5 dpi (P < 0.01 and P = 0.04, respectively), with no significant difference in either between the two groups at 1 dpi. Data for both are the mean ± SEM and represent two similar experiments, each with 5 mice/group/time.