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Murine model of Fusarium biofilm keratitis. Fusarium conidia were incubated 90 minutes with lotrafilcon A contact lenses, washed, and incubated a further 48 hours. (A) Fusarium hyphae associated with the contact lens (DIC microscopy). (B) After incubation with FITC-ConA, showing hyphae and extracellular matrix. (C) Murine model: 2-mm-diameter punch of a contact lens with attached Fusarium biofilm on the corneal surface of a C57BL/6 mouse. Original magnification: (A, B) ×400; (C) ×20.
