Figure 1.

Transgenic construct and resultant founders. (A) The BAC clone RP23–89F9 contained a fragment of mouse chromosome 8 (0.22 Mb) spanning the Grk1 gene with its exons (□), introns, and the full flanking sequences (solid line) inserted into the EcoRI site of pBACe3.6. The position of the 5′ PCR primer (filled arrow and arrowhead) and the 3′ primer (open arrow and arrowhead) sets are shown. The 5′ and 3′ primer sets amplified a 273- and 261-bp junctional insert–vector fragment, respectively. (B) PCR analysis of the genomic DNAs from mice generated by pronuclear injections of oocytes with a 5′ primer set. Bold arrow: the position of the band generated by amplification. The bottom band in each lane represents a possible primer dimer. Grk1⁺, Grk1 overexpressing mouse line 1, WT, transgene-negative wild-type mouse.