Figure 4.

Leukotriene synthesis by mouse retinal glial cells. (A) Methanol extracts of mouse retinal glial cells were analyzed by mass spectrometry. Mouse retinal glial cells produced LTB₄ in a dose-dependent manner after the addition of LTA₄. (A, inset) After the addition of 100 nM LTA₄, LTB₄ synthesis was enhanced under conditions of high glucose (HG) compared with physiologic glucose (NG, n = 3; *P < 0.02). (B) Western blot analysis of retinal glial cell lysates demonstrated a high glucose (HG)-induced increase in expression of LTA₄ hydrolase compared with the reference protein, β-tubulin, when compared with physiologic glucose (NG, n = 6; *P < 0.001).