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Confocal images of full-thickness constructs at 4 weeks. Images show the maximum projection of all the planes of focus for each sample. C or control (No TGF-β1) was compared with T1 (TGF-β1 present for the entire culture time). Fibrosis markers—(A, B) SMA, (C, D) type III collagen or Col III, and (E, F) EDA-Fn—were observed by indirect immunofluorescence. The expression of SMA, type III collagen, and EDA-Fn all increased when TGF-β1 was introduced into the system (T1). Bar, 50 μm.
