Figure 6.
Caffeine-evoked Ca2+ transients were enhanced after exposure to forskolin. (A) A sample record of [Ca2+]i for an arteriole which was exposed to caffeine (10 mM) under control conditions, after a 10-minute exposure to forskolin (10 μM), and finally, approximately 10 minutes after washout of forskolin. Changes in the fura-2 fluorescence ratio (ΔR) were normalized to the resting ratio in the same arteriole (R0). (B) Summary data for 12 arterioles showing the mean responses (±SEM) to each of the caffeine stimuli during the protocol shown in (A). *P < 0.05; **P < 0.01; versus control in both cases.