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. 2010 Feb;51(2):1198–1207. doi: 10.1167/iovs.09-4102

Figure 6.

Figure 6.

Analysis of retinal MMP-9 gelatinase activity after excitotoxic insult. Rats received intravitreal injection of vehicle (10 nmol glycine alone) or NMDA (20 nmol) plus glycine along with either scrambled peptide or P-IQACRG (150 pmol). Six hours after injection, retinas were harvested for gelatin zymography to assess MMP activity. (A) MMP-2 and -9 in tissue homogenates were affinity-precipitated with gelatin beads and separated by SDS-PAGE on polymerized gelatin. Gelatin digestion indicated MMP activity and was visualized as a clear band on dye-stained gels. Conditioned medium from the fibrosarcoma cell line HT1080, which is known to contain gelatinase activity, served as a positive control. (B) Quantitative comparison of gelatin zymography by densitometric analysis (mean value of eyes treated with scrambled peptide set at 100%). Statistical analyses revealed that P-IQACRG inhibited NMDA-induced MMP-9 activity (*P < 0.01 by t-test). Values are mean ± SEM (n = 5–6 for each group).