Figure 3.

Identification of the 70 kDa protein as albumin by MS/MS analysis and MASCOT search. (A) The amino acid sequence of serum albumin (Macaca mulatta) is shown at the top with bold regions indicating the eight tryptic peptides identified by LC/MS/MS analysis. The eight peptides are a part of the amino acid sequence of serum albumin. (B) An example spectrum of the peptide LVNEVTEFAK is shown. The spectrum shown in this example was obtained by LC/MS/MS analysis of the doubly charged [M + 2H⁺] ion.