Figure 4.

Confirmation that albumin is elevated in the retina of monkeys with experimental glaucoma by Western Blot analysis. Equal amounts of retinal protein extracts from control and glaucoma monkey eyes were separated by SDS-PAGE, transferred to nitrocellulose membranes, and probed with an antibody to human serum albumin. Each pair of blots contains sample from the control (first lane) and glaucoma eye (second lane) of the same monkey for each of seven monkeys. (A) Labeling for albumin is apparently greater in the retina of the glaucoma eye compared with the companion control eye. (B) Quantitative analysis of the relative change of albumin in the retina of glaucoma eyes to that of the fellow control eye for each monkey, after normalizing to β-actin, show increased amounts of albumin in all but one of the glaucoma eyes compared with control eyes.