Figure 8.

RT-PCR of apoptosis-related genes (n = 5/group/time/test). (A) Caspase mRNA expression was decreased in BALB/c FasL^−/− compared with WT mice Mφ after LPS treatment at all concentrations except 25 μg/mL (P = 0.01, P < 0.001, and P = 0.003, respectively). (B) Caspase 9 mRNA levels were lower in BALB/c FasL^−/− than in WT Mφ after LPS treatment at all tested concentrations except 100 ng/mL (P = 0.03 for all). (C) Mφ from BALB/c FasL^−/− mice expressed less BAX after LPS treatment at concentrations of 10 and 25 μg/mL compared with cells from WT mice (P = 0.008 and P < 0.001). No differences were detected at LPS concentrations of 100 ng/mL and 1 μg/mL between groups, and no differences were detected after PBS.