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. 2010 May;51(5):2537–2547. doi: 10.1167/iovs.09-4152

Figure 9.

Figure 9.

Real-time RT-PCR and ELISA for cytokine/chemokine expression (n = 5/group/time/test). (A) TNF-α mRNA expression was upregulated in BALB/c FasL−/− compared with WT Mφ at LPS at concentrations of 1 and 10 μg/mL (P = 0.04 and P = 0.03, respectively). No differences were detected at other concentrations or after PBS treatment. (B) TNF-α protein levels were elevated in supernatants of BALB/c FasL−/− compared with WT Mφ after LPS (all concentrations) but not after PBS (P < 0.001, P < 0.001, P = 0.03, and P < 0.001 respectively). (C) MIP-2 mRNA levels were elevated in BALB/c FasL−/− compared with WT Mφ after LPS at 100 ng/mL (P = 0.02). No differences were detected at other concentrations or after PBS treatment. (D) mRNA expression for IL-1β was increased in Mφ from BALB/c FasL−/− compared with WT mice after LPS treatment at 100 ng/mL and 10 μg/mL (P = 0.03 and P = 0.02, respectively) but not at other concentrations or after PBS. (E) IL-10 mRNA expression was decreased in LPS stimulated Mφ from BALB/c FasL−/− compared with WT mice at all concentrations except 100 ng/mL (P = 0.03, P < 0.001, and P = 0.01 respectively). No difference was detected between the groups after PBS. (F) No difference was detected for IL-12 mRNA expression after LPS treatment (all tested concentrations) or after PBS in Mφ from either group.