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. 2010 Mar 26;11(5):400–405. doi: 10.1038/embor.2010.37

Figure 2.

Figure 2

Cytolysin-mediated translocation is not dependent on pore formation but is enhanced by oligomerization. The ability of the SLO pore-forming mutants (Table 1) to translocate SPN into the A549 cell was analysed. The bars show the percentage of the total SPN expressed that was translocated into the A549 cytosolic fraction, after a 3.75 h infection. An ‘*' indicates that CMT is significantly lower than wild type (P<0.0001) and ‘**' indicates CMT is at levels higher than SLO− (P<0.005). The data represent the mean±s.e.m. values derived from at least three independent experiments. CMT, cytolysin-mediated translocation; SLO, streptolysin O; SPN, Streptococcus pyogenes nicotinamide adenine dinucleotide-glycohydrolase; wt, wild type.