Table 3.
Real-time PCR validation of stress-related and TCA cycle genes
| scFvD1.3 vs Skp/scFvD1.3 | scFvD1.3 vs FkpA/scFvD1.3 | |
|---|---|---|
| Gene | Real-time PCR expression ratio | |
| clpS | 1.6 | 1.9 |
| dnaJ | 2.1 | 3.0 |
| fkpB | 1.6 | 1.7 |
| grpE | 1.8 | 1.4 |
| hslR | 2.2 | 2.5 |
| ibpA | -1.1 | 1.4 |
| ybbN | 2.5 | 2.4 |
| sdhB | -2.1 | -2.7 |
| sdhD | -2.5 | -2.6 |
| sucD | -2.5 | -2.4 |
Quantitative real-time gene expression comparison of the wildtype scFvD1.3 over the chaperone co-expressing Skp/scFvD1.3 or FkpA/scFvD1.3 cells. Seven upregulated stress-related genes and three down-regulated TCA cycle genes from the microarray were validated with real-time PCR.