Figure 4.
Over-expressed Fry was found in the gaps between actin bundles. Panels A1-3 show a neuGAL4/UAS-myc-fry-GFP bristle with F-actin in red (Alexa 568 with phalloidin), Fry-GFP in green (enhanced by staining with an anti-GFP antibody) and merged image. Note both F-actin and Fry-GFP are in stripes. Higher magnification images of this bristle are shown in the B panels. Note that the Fry stripes are wider than the F-actin stripes. The intensity of the red and green staining across the bristle is shown in C. The red and green peaks and troughs coincide. An oblique cross section reconstructed from a stack of images (D) shows the offset between the red and green staining by an alternative approach. The stripes of Fry-GFP could be detected by in vivo imaging of bristles in actinGAL4/UAS-myc-fry-GFP; fry1/fry2 pupae (E).