Figure 8.

Trc amino acids important for the interaction with Fry. Panel A shows a diagram of the Trc protein and below in blue are the proteins (wild type and mutant) that were the initial set of deletion mutants analyzed. The interaction of these proteins with N-Fry in the yeast two hybrid system is shown. Deletion of the amino acids from 337-404 (in turquoise) resulted in a loss of the interaction while deletion of amino acids 404-464 (in orange) resulted in a stronger interaction. + indicates a positive interaction with N-Fry, and - no interaction (same meanings in B and C). Panel B shows a set of phosphorylation site mutations that were tested in the two hybrid system. The mutants did not differ from wild type Trc indicating phosphorylation of the Trc 453T did not regulate the interaction with N-Fry. In panel C a set of additional Trc deletion mutants were tested for their interaction with N-Fry in the two hybrid system. Deletion of the segment from 424 to 414 (colored in green) resulted in enhanced interaction while deletion of the segment in purple (337-350) resulted in a loss of the interaction. Panels D1 and D2 show the amino acids in these regions are are conserved in other NDR kinases.