Skip to main content
. 2010 May 12;5(5):e10600. doi: 10.1371/journal.pone.0010600

Figure 4. Transcriptional activity of the D1 and D2 putative promoters in e12.5 transgenic embryos.

Figure 4

(A) A schematic representation of the Hoxa5, Hoxa6 and Hoxa7 genes along the HoxA cluster. The proximal promoter P and the two distal promoters D1 and D2 are indicated. (B) Detection of β-galactosidase activity in D1-lacZ transgenic embryos in presence of the mesodermal enhancer sequence (MES) indicates that the 4 kb-DNA region encompassing the putative Hoxa6 promoter (D1) can drive Hox-like expression along the antero-posterior axis. (C) As well, a 4 kb-DNA fragment containing the D2 putative promoter region of the larger transcripts possesses a similar transcriptional activity. B, BglII; H, HindIII; N, NruI; RI, EcoRI; Xh, XhoI.